Journal: Communications Biology

Article Title: Slow viral propagation during initial phase of infection leads to viral persistence in mice

doi: 10.1038/s42003-021-02028-x

Figure Lengend Snippet: a , b C57BL/6 mice were infected with 2 × 10 4 pfu LCMV-WE (WE), and LCMV-Docile (Docile). At day 2 post-infection, spleen tissues were stained for LCMV antigen NP, CD169, CD11c, and B220 ( a ) or CD90.2, F4/80, and Ly6c ( b ) (representative sections are shown ( n = 4) scalebar = 20 µm). C57BL/6 mice were infected with LCMV-WE (WE), LCMV-Docile (Docile), or WE together with Docile (WE + Docile). c , d At day 2 post-infection, numbers of c splenic cDC (CD11c + MHC-II + ) and pDC (B220 + Siglec-H + ) were determined ( n = 9, gating strategy is shown in Supplementary Fig. ). d Co-stimulatory molecules CD40, CD80, and CD86 were measured on splenic cDC ( n = 6). e Serum IFN-α concentrations were determined at the indicated time post-infection ( n = 9). f Serum IL-6, g serum TNF-α, h serum IL-1β concentrations were measured at the indicated timepoints ( n = 5). (Error bars show SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, and ns indicates statistically not significant between the indicated groups).

Article Snippet: Antibodies against CD169 (Bio-Rad), CD11c, B220, CD90.2, F4/80, Ly6C (ebioscience), donkey-anti-rat secondary antibody (jackson immunoresearch), and self-made anti-LCMV monoclonal antibody (Clone: VL-4), were used.

Techniques: Infection, Staining